# ISO 7251:1993 — Withdrawn and superseded ISO standards For ISO 7251:1993, item title is Microbiology — General guidance for enumeration of presumptive Escherichia coli — Most probable number technique; status code is 9599; successor is ISO/CD 16649-3 | ISO 7251:2005; withdrawn / published date is 1993-12-23, recorded from its source on 2026-08-05. - **Identifier:** ISO 7251:1993 _(verified: appears in the quote below)_ - **Item title:** Microbiology — General guidance for enumeration of presumptive Escherichia coli — Most probable number technique _(verified: appears in the quote below)_ - **Status code:** 9599 _(verified: appears in the quote below)_ - **Successor:** ISO/CD 16649-3 | ISO 7251:2005 - **Withdrawn / published date:** 1993-12-23 _(verified: appears in the quote below)_ ## What the source says > 13905,IS,,ISO 7251:1993,Microbiology — General guidance for enumeration of presumptive Escherichia coli — Most probable number technique,Microbiologie — Directives générales pour le dénombrement d'Escherichia coli présumés — Technique du nombre le plus probable,1993-12-23,2,['07.100.01'],ISO/TC 34/SC 9,9599,[13904],"[31241,34568]","['en','fr']",7,"
Gives general guidelines for the enumeration of presumptive Escherichia coli in products intended for human consumption or feeding of animals, by means of the liquid-medium culture technique and calculation of the most probable number after incubation at 35 °C or 37 °C (this temperature forming the subject of agreement between the parties concer ## Source - https://isopublicstorageprod.blob.core.windows.net/opendata/_latest/iso_deliverables_metadata/csv/iso_deliverables_metadata.csv Last verified: 2026-08-05. Review by: 2027-02-01. Part of [Withdrawn and superseded ISO standards](https://referencesource.org/iso-standard-supersessions/).