# ISO 4052:1983 — Withdrawn and superseded ISO standards For ISO 4052:1983, item title is Coffee — Determination of caffeine content (Reference method); status code is 9599; successor is ISO/PWI 4052 | ISO/WD 4052; withdrawn / published date is 1983-06-01, recorded from its source on 2026-08-05. - **Identifier:** ISO 4052:1983 _(verified: appears in the quote below)_ - **Item title:** Coffee — Determination of caffeine content (Reference method) _(verified: appears in the quote below)_ - **Status code:** 9599 _(verified: appears in the quote below)_ - **Successor:** ISO/PWI 4052 | ISO/WD 4052 - **Withdrawn / published date:** 1983-06-01 _(verified: appears in the quote below)_ ## What the source says > 9756,IS,,ISO 4052:1983,Coffee — Determination of caffeine content (Reference method),Cafés — Détermination de la teneur en caféine — Méthode de référence,1983-06-01,1,['67.140.20'],ISO/TC 34/SC 15,9599,,"[33995,24198]","['en','fr']",6,"

The method consists in extracting of the caffeine from a test portion, in an ammoniacal medium, successive purifying, with diethyl ether, on two chromatographic columns, the first in an alkaline medium, the second in an acid medium, followed by elution by chloroform. Spectrometric measurement of the eluate at the wavelength of maximum absorbance (in the ultraviolet region). A first figure shows examples of chromatographic columns. A second figure gives an e ## Source - https://isopublicstorageprod.blob.core.windows.net/opendata/_latest/iso_deliverables_metadata/csv/iso_deliverables_metadata.csv Last verified: 2026-08-05. Review by: 2027-02-01. Part of [Withdrawn and superseded ISO standards](https://referencesource.org/iso-standard-supersessions/).