# ISO 3594:1976 — Withdrawn and superseded ISO standards

For ISO 3594:1976, item title is Milk fat — Detection of vegetable fat by gas-liquid chromatography of sterols (Reference method); status code is 9599; successor is ISO/WD 3594; withdrawn / published date is 1976-12-01, recorded from its source on 2026-08-05.

- **Identifier:** ISO 3594:1976 _(verified: appears in the quote below)_
- **Item title:** Milk fat — Detection of vegetable fat by gas-liquid chromatography of sterols (Reference method) _(verified: appears in the quote below)_
- **Status code:** 9599 _(verified: appears in the quote below)_
- **Successor:** ISO/WD 3594
- **Withdrawn / published date:** 1976-12-01 _(verified: appears in the quote below)_

## What the source says

> 9005,IS,,ISO 3594:1976,Milk fat — Detection of vegetable fat by gas-liquid chromatography of sterols (Reference method),Matières grasses du lait — Détection des matières grasses végétales par chromatographie en phase gazeuse des stérols (Méthode de référence),1976-12-01,1,['67.100.10'],ISO/TC 34/SC 5,9599,,[9006],"['en','fr']",5,"<p>The method consists in preparing of sterol digitonides and dissolving in a mixture of formamide and dimethylformamide, extraction of the liberated sterols with pentane, separation of the sterols using a suitable gas chromatograph. If, on the chromatogram, a peak with the retention time of beta-sitosterol is obtained, the presence of vegetable fat in the sample is

## Source

- https://isopublicstorageprod.blob.core.windows.net/opendata/_latest/iso_deliverables_metadata/csv/iso_deliverables_metadata.csv

Last verified: 2026-08-05. Review by: 2027-02-01.
Part of [Withdrawn and superseded ISO standards](https://referencesource.org/iso-standard-supersessions/).