# Single file NIfTI format, used extensively in biomedical imaging. — file signature 6E 2B 31 00 at offset 344 For Single file NIfTI format, used extensively in biomedical imaging., hex signature is 6E 2B 31 00; offset is 344; extension is nii, verified against its source on 2026-09-22. - **Hex signature:** 6E 2B 31 00 _(verified: appears in the quote below)_ - **Offset:** 344 _(verified: appears in the quote below)_ - **Extension:** nii _(verified: appears in the quote below)_ - **Format:** Single file NIfTI format, used extensively in biomedical imaging. _(verified: appears in the quote below)_ ## What the source says > 6E 2B 31 00 | n+1 | 344 | nii | Single file NIfTI format, used extensively in biomedical imaging. | ## Source - https://en.wikipedia.org/wiki/List_of_file_signatures Last verified: 2026-09-22. Review by: 2027-09-22. Part of [File format magic numbers](https://referencesource.org/file-format-signatures/).